Third Time's the Charm? Or Not...

After yesterday's disappointment at the failed Pulsed-Field, I prepared for the chamber test today slightly more hopeful. At least, I thought, we could determine what was actually causing the problems instead of just being in the dark on the matter. I set up one large and one small gel, one for each chamber. It was quite a busy work day, actually, with all that I had to do. In addition to the Pulsed-Field, I ran gels to check the PCR I had done the previous day that would finish off the tetA part of my experiment. It turns out that all our weak positives were negative, so that was fine. Glad to have a bit of it done already. Regardless, I didn't end up leaving until 5:15, and that was only because Sandra offered to put the second gel on the machine for me.


I was glad that she did, because it allowed me to go into town to meet up with Oein for a snack. We ran into a few other friends as well, so that was fun. After that, I went back home and relaxed for a little bit before getting word that everyone was going out for the evening.

We started at our usual spot, Bierhaus, where everyone had a grand ole time. The girls especially, since they managed to procure some pretty cool glasses. From there, we broke off into groups and headed up to Eyre Square, deciding to go to Bar 903. They had a cover band playing, so that made the night a bit more fun. We got to hear some excellent old songs (like "Summer of '69" and "Hotel California") as well as some newer ones (The Killers - "All These Things That I've Done"). Fun stuff!


Overall we just had a good time, goofing off and singing our heads off. There was a bit of dancing mixed in there as well. We stayed there for a long time, until they pretty much kicked us out (nicely, of course). The rest of the group went ahead to Corrib Village, while Ruth, Richard, and I ventured down by the river to grab some things that they'd left there earlier. It was an amusing walk, but we had fun. We took a cab home after that, and hung around the apartments waiting for everyone else to get back since they walked.


After about 15 or 20 minutes, people started streaming back in to the complex, and we had a good time hanging out and joking around. Some of the guys decided it would be fun to "box", or something like that, but we stopped it before they crossed any lines. It was amusing to begin with. As the others put in a movie to watch, I left and came back to my apartment to crash, since I was exhausted. I really hope my gel works tomorrow...and that I don't have nightmares about it tonight! (because that would be sad)